Confocal analysis of dermal fibroblasts after heat shock stress (progeria)

Autor:

Mauro Paradisi , Dayle McClintock , Revekka L Boguslavsky , Christina Pedicelli1 , Howard J Worman and Karima Djabali

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Confocal analysis of dermal fibroblasts after heat shock stress. Cells were incubated at 45°C for 30 minutes, then either immediately fixed (time 0) or allowed to recover for 24 or 48 hours at 37°C. Cells were processed for indirect immunofluorescence labelling using anti-lamin A/C antibodies (panel A, a to f), anti-lamin B1 antibodies (panel B, g to r) and anti-vimentin antibodies (panel B j to i and p to r). Control and HGPS fibroblasts are indicated. (A) Fibroblasts were immunostained with anti-lamin A/C antibodies. Note the increased number of dysmorphic nuclei in HGPS fibroblasts compared to control 24 hours after recovery from heat shock. (B) Fibroblasts were immunostained with anti-lamin B1 and vimentin at times indicated after heat shock. Bar, 10 μm. Paradisi et al. BMC Cell Biology 2005 6:27 doi:10.1186/1471-2121-6-27

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© 2005 Paradisi et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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CC BY 2.0

Credit:

Dermal fibroblasts in Hutchinson-Gilford progeria syndrome with the lamin A G608G mutation have dysmorphic nuclei and are hypersensitive to heat stress

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